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Melody
Nov 23rd 2006, 05:19 AM
Intro. to Live Food Culturing


Following is a brief introduction to culturing the most common live foods. Further research into methods is always recommended as they do vary. I haven't cultured all of these live foods, so you'll often be reading what I've picked up in my studies of the topic.

Containers: Recycled, disposable or Dollar Store containers will all work for worm cultures. Remember to punch small holes in the lid for air and especially to avoid explosions if yeast is a part of the culture. If you have more than one culture, it can't hurt to label the container.

Storage: Some cultures are more sensitive to heat than others, but all should be kept in a cool location (low 70's as a rule). You can place the culture in a styro box in the Summer with a few ice cubes in a bag, a lunchbox cold pack, a small bottle of frozen water, etc.

Start new cultures on a regular basis to ensure a stable and prolific population.


Green Water - Tiny Newborn or Culture Food

Green Water is suspended algae.

Culture: The most common method is to add grass clippings to water, add a bit of plant ferts and place the mixture in a well-lit location - preferrably in front of a window or outside in the Summer. Be careful with the ferts if you intend to feed the culture directly to the tank of fry as it can cause a build-up of phosphates

You can also use the 'messier' fish or snails to feed the Greenwater culture in lieu of ferts. I prefer snails because they also contribute infusoria to the mix, resulting in a more nutritionally complete cocktail, and keep the sides of the tank clean so the light is allowed through. Since I raise snails, it is a wonderful place for the babies to grow while contributing to the system. Do not use too many though, or the culture will smell horrible.


Infusoria - Newborn Food
Some fish are born amazingily small, such as Neon Tetras and some Rainbowfish species. To feed these fish after they are free-swimming, you may require Infusoria. The great thing about Infusoria cultures, is that you don't need to obtain a starter culture.

Infusoria is a mix of various tiny creatures used to feed the smallest fry. Basically, it is a mix of mini Rotifiers. To start an Infusora culture, you will need a jar and food. Place a bit of your food of choice into a jar and fill it up with aquarium water. A couple of days later you may notice foggy water, at which point you can remove a small amount of water and add more aquarium water. Within a week, you should have clear water and begin to see little critters in there.

Food: Infusorians feed on bacteria created by decaying organics. It doesn't take much to feed them and the less food there is, the less smell there is. I have had them show up in a stagnant small tank that I forgot about, so its not rocket science. You can use established tank water/mulm, aquarium plants, hay, lettuce, etc.

Note: Applesnails 'produce' infusoria in their feces - they work with their digestive system to digest food. Keeping them in your fry tanks ensures a constant supply of infusorians. They'll also serve as janitors while they're at it.


Paramecium - The Other White Meat

Often lumped in with Infusoria, Paraecium is cultured quite differently. So incredibly tiny, they should not be raised in tank water or they risk being eaten by other organisms. The water should be boiled or distilled. Paramecium will live for quite sometime in your tank.

Food: Paramecium feeds on bacteria. Slightly boiled kernals of wheat, Liquifry, corn husks, etc will facilitate the bacteria growth. Add the food and 1/4 tsp of brewer's yeast to the jar of water, stirring well. Add your Paramecium culture. Cover and store in a cool place.

Harvest: You should be able to harvest in a couple of weeks or so. Use a large eye-dropper or turkey-baster to harvest the Paramecium and squirt it into the fry tank.


Vinegar Eels - Tiny Newborn Food / Transition Food

Vinegar Eels can be used as a transition food between micro-cultures, such as Infusoria, and BBS. A big advantage of Vinegar Eels is that they can stay alive in freshwater for weeks.

Culture: In your container of choice, add 1/4 - 1/2 of an apple. Fill the container about 3/4 of the way with a 50/50 mix of water and Apple Cider Vinegar.

Harvest: The Vinegar Eels will be ready to harvest in about a month. Use a turkey baster or eye-dropper to bring the Eels out of the culture and squirt them into a coffee filter placed in a funnel or small strainer. Gently rinse the Eels a couple of times in the filter, and then swish the filter into a container of water. Feed using a turkey baster or eye-dropper. For an alternate method of harvest, check out this (http://www.livefoodcultures.com/vinegar_eel_harvesting.html) illustrated article.


Microworms - Newborn / Transition Food

Microworms are a food between the size of Green Water and Brine Shrimp. They are often recommend for Rainbowfish as a starterfood, but any newborn will enjoy them, in my experience, including small newborn Livebearer fry.

Culturing Microworms couldn't be easier by comparison to other live foods. Prepare some oatmeal and/or baby cereal to keep them in. After experimenting I decided on a mixture of both. Top it off with a bit of active yeast, and mix it all up with some water until it is very moist. Add your starter culture, place a ventilated lid on the container, and place in a cool area. In a week or two there should be plenty of wiggly worms on the sides of the container. Supplement with finely ground seaweed/algae if you wish.

Harvest: Use a cotton swab, popsicle stick, etc, to scrape the worms off the sides. You can either drop them into water and extract them with an eye-dropper, or feed directly. Be careful not to add the food mixture with them though, or you'll foul the tank.


BBS - Baby Brine Shrimp (Artemia) - Fry food

This is the live fry food that most are familiar with. BBS are shrimp larvae which is hatched into salt water and is most nutritious within eight hours of hatching.

Culture: Culturing isn't difficult, but it is a little more involved than the other starter foods. You can purchase BBS hatcheries or build your own - there are various DIY hatcheries at the tips of your Google fingers, one of which can be found here (http://www.petfish.net/kb/entry/15/92/). The basic items include an air pump, airline tubing, your hatchery of choice, non-iodized salt, Brine Shrimp eggs and a Brine Shrimp net. Measurements may vary, but basically you add 1/2L of water, get it bubbling using the pump/airline tubing, add 1/4 tsp of Brine Shrimp eggs & 1 TBSP of non-iodized salt.

Harvest: Brine Shrimp eggs take about 24 hours to hatch. When they are ready, stop the pump and syphon the shrimp into a brine shrimp net or cheesecloth. You use the syphon to both remove and separate the Shrimp from the hatched eggs. Empty the BBS into a container of fresh water and use an eye-dropper or turkey baster to feed.


Gammarus: Junior or Small Fish Food

http://i48.photobucket.com/albums/f227/canadiansinternetdotcom/MM-GammaruShrimp2.jpg


Gammarus amphipods are a very small shrimp-like creature commonly found in freshwater. They are very easy to culture, not in the least sensitive or demanding in my experience. I have a 10G swarming with them as we speak and I've totally ignored them in regards to special care. They like to cling to plants, including floaters, or they hide in gravel, under driftwood, etc. The live plants probably supplies them with rotting plant matter & fungi. They will also eat seaweed powder which can be used to gutload them. Shrimp pellets and other fish food is also eaten. They're high in calcium and a good source of roughage.


Grindle Worms: Junior or Small Fish Food

These worms have long been used as a transition from the usual fry foods to larger live foods or for smaller adult fish, such as Endler's Livebearers or Killifish.

Bedding: Coconut Fiber (Check your pet store reptile section) or peat moss. The latter may prove to be too acidic, in which case you can mix it with the Coconut Fiber or sterilized gardening soil with no additives. The bedding should be damp. Place the bedding into a container and add your starter culture.

Food: Dampen a very small amount of multi-grain or oatmeal baby cereal and sprinkle it on top, preferrably in one small area of the surface.

Harvest: The worms will be massed in the feeding area. When you want to feed them to your fish, simply remove a portion and feed. If you find that they have too much bedding coming with them, they may be rinsed. Alternately, you can lightly spray a piece of glass or plastic with dechlorinated water, dust with food and lay it in the container. You can use a small rock to keep one side slightly elevated and out of the dirt for a cleaner harvest. When you see a bunch of worms on the glass/plastic, you can use a cotton swab or popsicle stick, etc to scrap the worms off for feeding.


Daphnia - Nature's system restore

There are a large number of Daphnia species, varying in size, with some species being smaller than BBS at birth. The most common species appear to be Pulex and Moina, with the latter being smaller. They are most often fed to small fish as a conditioning food, or to clean out their digestive system / treat constipation. Daphnia can be somewhat sensitive to environmental changes and water quality, so this method of producing live food takes a bit of dedication.

Culture: Many people culture their Daphnia in established Green Water cultures, which keeps the water pure and feeds the Daphnia. Otherwise, you may use a tank filled to about the halfway point with water treated to remove metals, the larger the tank the better. You can culture Daphnia in smaller tanks with diligence. The Daphnia starter culture should be temp acclimated and then added by slowly immersing the culture into the water.

Food: Green Water is the food of choice for Daphnia most of the time. When that isn't practical, you can use small fry foods, tiny amounts of pre-mixed yeast, very finely ground seaweed/algae or seaweed paste, or organic matter.

Harvest: Daphnia is best harvested daily once established.

Note: I recommend further reading on this one - it is much more involved than I have covered here as an introduction.


Whiteworms - Adult food or large junior fish

Whiteworms can survive in water for a few days. They will burrow under gravel, however, so they are recommended for either bare or lightly gravelled tanks, or tanks with 'digging' scavengers, such as Loaches. You don't want a bunch of dead worms decaying in your gravel.

Culture: Whiteworms should have a sterile, moist bedding and be kept in a well-ventilated container. They require very cool conditions, preferrably in the 60's.

Food: Couldn't be easier - Cat/Dog food (probably not the best choice as the mammal fat may reach the fish through the gut of the worms), ground seaweed/algae, ground powdered or mashed vegies, fish food, bread, baby cereal.... pretty much anything within reason as long as it is moist.

Harvest: Place glass or plastic over a shallow hole in the bedding in which you'll place the worm food. The worms will gather there, facilitating an easy harvest. Rinse and serve.


The Culture of Earthworms For Fish Food (http://edis.ifas.ufl.edu/FA016)


Culturing Mosquitos/Larvae (http://www.jove.com/index/details.stp?ID=221) - This is a scientific guide to rearing Mosquitos/Larvae, complete with video. Theoretically, if you gather larvae, raise them to breeders and then breed them, you could have a source of disease-free food which many fish thrive on in the wild.


My friend Mike Hellweg has written a fantastic book, Culturing Live Foods: A Step-By-Step Guide to Producing Food for Your Home Aquarium (http://www.amazon.ca/gp/product/0793806550?ie=UTF8&tag=canadiansinte-20&linkCode=as2&camp=15121&creative=390961&creativeASIN=0793806550). It covers the traditional and newer cultures maintained in the aquarium trade.


Gut-loading (Bio-Encapsulation): The term 'gut-loading' refers to feeding the culture highly nutrious food and immediatly feeding the loaded live food to your fish. This can vastly improve the nutritional content of the live foods, while also providing a more specialized diet for fish, such as herbivores. Worms are great candidates for gut-loading. You can feed lightly moistened powdered seaweed or vegetables, finely crushed, high quality fish foods and baby cereal, to name a few. Providing complete nutrition for your fish will encourage proper development.


Where to obtain starter cultures: Your local fish club is a great place to find cultures. You can also swap cultures (http://www.canadianaquariumconnection.com/forum/showthread.php?t=6029) with other hobbyists. Keep an eye on the auction (http://www.canadianaquariumconnection.com/ClassAuction/) for cultures listed there as well. :SharkSharkShark!:


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Soggybottom
Dec 5th 2006, 05:23 PM
If anyone wants to really get into this, I have a manual on home-size to commercial-size culture of just about everything that swims and is eaten by fish. It has figures on optimum levels of everything...I know the publishers aggressively defend the copyright though so I can't post anything. Just let me know if you want any technical info and I'll PM.

I've tried a few different cultures and what I've found escpecially with daphnia is that if you want a decent size, intensive culture, you are basically running another fish tank with all the same maintenance issues. It is not really a cheap or easy way to feed fish, just a healthy and delicious way :)

Paramecium - The Other White Meat Lol

Good thread Melody!

Melody
Jan 19th 2007, 05:20 AM
I forgot all about this list and thought you might enjoy it. Wanders a bit off topic, gets some spam and isn't terribly busy, but good information comes up on a regular basis. The link goes to the list homepage, so you can check out the archives before subscribing if you want a preview.

Live Foods Mailing List by FINS (http://fins.actwin.com/live-foods/)

From the website:

The live foods mailing list is intended to be a medium for exchange of information about all aspects of growing live foods for use within that aquarium hobby. Postings on the culture and suitability of various live foods and food preparations are welcome. Topics of discussion include (but are not limited to):

The live food itself (identification, culturing, etc)
Media and foods for raising live food
Nutritional value of various live foods and their suitability for aquarium use
Potential pitfalls of various live foods
Trades/exchanges between hobbyists

Melody
May 15th 2007, 02:08 PM
With our appreciation to the U of F for this educational article. CACDecapsulating Brine Shrimp EggsCraig Watson and Roy P.E. Yanong2 (http://edis.ifas.ufl.edu/FA023#FOOTNOTE_2) IntroductionBrine shrimp eggs are used throughout the world as a food for small fish in hatcheries. These eggs are really cysts which, if they are kept dry, can remain dormant for years before hatching. As soon as the eggs are exposed to water, the hatching process begins. When hatching brine shrimp eggs, we not only produce baby brine shrimp, but also the empty shells out of which they came, along with unhatched eggs. These are mixed in the hatching jar. The unhatched eggs and shells from the hatched eggs, must be separated from the baby brine shrimp since they are not digestible if eaten by small fish. If a small fish eats just a few of these shells or unhatched eggs, its intestinal tract may be blocked causing death. The process of separating the shells and unhatched eggs from the baby brine shrimp is quite time consuming, and sometimes hard to do effectively. A process that is used in many hatcheries involves removing the outer layer (shell) of the eggs (decapsulating) with chlorine (household bleach), leaving the unhatched baby brine shrimp protected in a membrane. Besides making the harvest of the hatched brine shrimp easier, this process also:

Essentially sterilizes the eggs which may have disease organisms on the outer layer of the egg.
Can produce a higher percentage of hatching, since the brine shrimp no longer have to break through the hard outer layer of the egg.
Allows you to feed even the unhatched eggs to fish, since the undigestible outer layer of the egg is no longer present.Decapsulated eggs can be hatched immediately after treating them with chlorine, or can be stored in a saturated brine solution in the refrigerator for up to two months before hatching. This allows you to decapsulate large quantities of eggs, store them, and use small portions as needed. The brine solution dehydrates the eggs, effectively stopping the hatching process. The eggs will hatch normally when placed in a hatching solution of lower salinity water.



Procedure Note: The following is the procedure for decapsulating one pound of eggs. The amounts of water and Household bleach (chlorine) can be changed accordingly if you are working with more or less eggs. You will need the following items:

A 3-gallon container with clear sides
1 pound of brine shrimp eggs
1 gallon of non-fragranced household bleach (5% chlorine)
Brine shrimp net or filter
Saturated brine solution** In 1 gallon of water, dissolve salt until no more can be dissolved and salt remains in the bottom of the container.



Steps in Process

Soak 1 pound of eggs in 1 gallon of fresh water for 1 hour. Gently aerate the eggs. Periodically check to make sure that the eggs are not sticking to the sides of the container, above the water line.
After the eggs have soaked in fresh water for 1 hour, add 1 gallon of non-fragranced liquid household beach (5% chlorine). Decrease the aeration to avoid foaming, while still mixing the eggs in solution.
The eggs will turn white and then orange, and start to settle to the bottom. As soon as all of the eggs are orange, pour the contents through a brine shrimp net (or filter), and rinse in fresh water. Continue to rinse until all traces of bleach odor are gone. The time it takes for all the eggs to be decapsulated will vary with the type of eggs being used, so it is more important to observe the color change than to watch a clock.
Decapsulated eggs can be fed directly to fish that will eat them, or they can be hatched before feeding them to fish. Any eggs that you wish to store should be covered with the saturated brine solution, and stored in a refrigerator until needed.Hatching Decapsulated Eggs



By removing the outer layer of the eggs, you will reduce their buoyancy, causing them to sink in water. This is important since a hatching system that works well for eggs with their capsules on, may not work for decapsulated eggs. Hatching containers should have steep sides to avoid the eggs settling on the sides, and aeration should come from the extreme bottom of the container. Cylindrical tanks with a steep cone in the bottom are ideal hatching containers. Salinity for hatching should be around 20 parts per thousand which is equal to 1 pound of salt for each 6 gallons of water. Using a strong light over the top of the tank also helps the hatching process. Decapsulated eggs hatch in 24-48 hours. The entire contents of the hatching container can then be drained through a brine shrimp net or filter and fed to your fish. Special NoteBrine shrimp eggs come from many different sources. Some will react differently to this treatment, with some eggs taking longer than others to turn orange in the chlorine solution. However, the basics described above will work for any type of brine shrimp eggs. Footnotes1. This document is Fact Sheet FA-18, one of a series from the Department of Fisheries and Aquatic Sciences, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. First published: June 1990. Reviewed: December 2002. Please visit the EDIS Web Site at http://edis.ifas.ufl.edu. 2. Craig Watson, Director, and Roy P.E. Yanong, Assistant Professor, UF/IFAS Tropical Aquaculture Laboratory, Ruskin, FL 33570, Department of Fisheries and Aquatic Sciences, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, FL 32611. The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations. For more information on obtaining other extension publications, contact your county Cooperative Extension service. U.S. Department of Agriculture, Cooperative Extension Service, University of Florida, IFAS, Florida A. & M. University Cooperative Extension Program, and Boards of County Commissioners Cooperating. Larry Arrington, Dean.Copyright InformationThis document is copyrighted by the University of Florida, Institute of Food and Agricultural Sciences (UF/IFAS) for the people of the State of Florida. UF/IFAS retains all rights under all conventions, but permits free reproduction by all agents and offices of the Cooperative Extension Service and the people of the State of Florida. Permission is granted to others to use these materials in part or in full for educational purposes, provided that full credit is given to the UF/IFAS, citing the publication, its source, and date of publication.

Katalyst
Sep 26th 2008, 01:44 AM
I have referred to this thread countless amounts of times & especially now with my newly hatched betta fry. I'm giving this thread a bump for any new member that may have missed it, well worth the read even after the umteenth time! :yes:

Melody
Sep 26th 2008, 09:57 AM
I haven't cultured everything in the post but there's some good info out there and in various books, so I gathered it up. If anyone has further info based on experience, by all means share it. :yes: There's also a copy of this thread in the articles section if ever someone wants to link to a clean thread without my ramblings in it....lol.

psychoactive
Dec 31st 2008, 12:03 AM
Nice! very interesting stuff i like the part that you added about the brine shrimp at the end it got me interested in giving it a try actually, very useful info.